Fig. 4

miR-424 is transferred from MSCs to ovarian cancer cells by EVs. a The expression of miR-424 in MSCs and MSC-derived EVs after treatments with miR-424 mimic and inhibitor as well as their NCs. b The uptake of EVs by ovarian cancer cells observed under a confocal fluorescence microscope (scale bar = 50 μm). The ovarian cancer cells SKOV-3 used for the following detections were co-cultured with MSCs, EVs-depleted MSCs, and MSCs treated with miR-424 mimic or miR-424 inhibitor as well as their NCs. c The expression of miR-424 and MYB in ovarian cancer cells detected by RT-qPCR. d The protein expression of MYB in ovarian cancer cells examined by Western blot analysis, with protein bands assessed. *p < 0.05 compared with MSCs, ^#p < 0.05 compared with mimic NC and MSCs mimic NC treatments; & p < 0.05 compared with inhibitor NC and MSCs inhibitor NC treatments. The data above were measurement data and were presented as sample mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA. The cell experiment was repeated 3 times independently