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Table 1 Primers sequences of selected circRNAs and internal reference for reverse transcription-quantitative polymerase chain-reaction (RT-qPCR) validation

From: Circular RNA expression profiles and features in NAFLD mice: a study using RNA-seq data

Forward Sequence (5′-3′) Name Reverse Sequence (5′-3′) Length
5′-GCTTCTAGGCGGACTGTTAC-3′ β-actin-R1 5′-CCATGCCAATGTTGTCTCTT-3′ 100 bp
5′-GCTCCTGGGAAGGTGACATC-3′ mmu_circ_0015959-R2 5′-CCTGAGGAGTTTCCTGGAAG-3′ 144 bp
5′-TGTGGCCGATTCTTTGCATT-3′ mmu_circ_0010514-R2 5′-CATCATGTCAACCTTGAGGG-3′ 109 bp
5′-CTTAGATCAGCCGTGTTGTG-3′ mmu_circ_0004580-R2 5′-ACAGTCCCATTAAGCCTTGC-3′ 144 bp
5′-ATAGATGGCTGGGGCTTTGG-3′ chr7_82671604_82674582-R1 5′-GCGAACCGCTGTTGATACTT-3′ 100 bp
5′-GCGACTCAGACACAGATCCA-3′ chr17_66049785_66053091-R1 5′-ACTGCTGTCACTGTCAGAAT-3′ 126 bp
5′-AACACTCTGCACGGGTCAAG-3′ chr4_46734057_46749582-R1 5′-TCCGAAGCTGCTCTAGAATG-3′ 203 bp
5′-GAACGTCCTATATCATTAGGG-3′ chr11_94036599_94048558-R4 5′-TTCAAGTCTGCTGACTTCAG-3′ 161 bp
5′-CATGAACTGCAGGGCTGAAC-3′ chr5_118593332_118593570-R1 5′-GCAGACAGCGGATGAAACTT-3′ 134 bp