Table 1 Primers sequences of selected circRNAs and internal reference for reverse transcription-quantitative polymerase chain-reaction (RT-qPCR) validation
From: Circular RNA expression profiles and features in NAFLD mice: a study using RNA-seq data
Forward Sequence (5′-3′) | Name | Reverse Sequence (5′-3′) | Length |
|---|---|---|---|
5′-GCTTCTAGGCGGACTGTTAC-3′ | β-actin-R1 | 5′-CCATGCCAATGTTGTCTCTT-3′ | 100 bp |
5′-GCTCCTGGGAAGGTGACATC-3′ | mmu_circ_0015959-R2 | 5′-CCTGAGGAGTTTCCTGGAAG-3′ | 144 bp |
5′-TGTGGCCGATTCTTTGCATT-3′ | mmu_circ_0010514-R2 | 5′-CATCATGTCAACCTTGAGGG-3′ | 109 bp |
5′-CTTAGATCAGCCGTGTTGTG-3′ | mmu_circ_0004580-R2 | 5′-ACAGTCCCATTAAGCCTTGC-3′ | 144 bp |
5′-ATAGATGGCTGGGGCTTTGG-3′ | chr7_82671604_82674582-R1 | 5′-GCGAACCGCTGTTGATACTT-3′ | 100 bp |
5′-GCGACTCAGACACAGATCCA-3′ | chr17_66049785_66053091-R1 | 5′-ACTGCTGTCACTGTCAGAAT-3′ | 126 bp |
5′-AACACTCTGCACGGGTCAAG-3′ | chr4_46734057_46749582-R1 | 5′-TCCGAAGCTGCTCTAGAATG-3′ | 203 bp |
5′-GAACGTCCTATATCATTAGGG-3′ | chr11_94036599_94048558-R4 | 5′-TTCAAGTCTGCTGACTTCAG-3′ | 161 bp |
5′-CATGAACTGCAGGGCTGAAC-3′ | chr5_118593332_118593570-R1 | 5′-GCAGACAGCGGATGAAACTT-3′ | 134 bp |