Fig. 2

Analyses of cell viability, apoptosis rates and population doubling. a Histograms of the percentage of viable cells after thawing, measured with trypan blue exclusion assay. b Population doubling (PD) calculated at Days 2, 5 and 7 of culture post-thawing. c Representative cytofluorimetric plots of apoptosis analyses and relative results expressed as percentages. Viability (V), early apoptosis (EA), late apoptosis (LA), and necrosis (N) of cells stored in LN₂ and dry ice were analysed immediately after cell thawing (Day 0) or after one week in culture (Day 7). Data are expressed as mean ± SD of three independent experiments. Statistical analysis was performed with GraphPad Prism software and Student’s t-test did not evidence any statistical difference