Fig. 6

Impact of inhibiting HDL carbamylation or NF-κB activation on monocyte adhesion and adhesion molecules expression. a, b HUVECs were treated with C-HDL (50 μg/mL), C-HDL (100 μg/mL) glycylglycine-incubated HDL (100 μg/ mL) for 6 h, then co-cultured with monocytes for 1 h. Control group was treated with sodium chloride. Adherent monocytes were counted on 5 randomly selected fields at 4 × magnification, with typical photographs taken. c, d Representative immunoblots and quantitative analysis of protein level of ICAM-1, VCAM-1, p65, and phosphorylation of p65. (**P < 0.01 compared with control, ^##P < 0.01 compared with glycylglycine-incubated HDL). e, f HUVECs with and without pretreatment of 5 mg/mL (-)- DHMEQ were exposed to C-HDL for 6 h (100 μg/ mL), then co-cultured with monocytes for 1 h. Control group was treated with sodium chloride. Adherent monocytes were counted on 5 randomly selected fields at 4 × magnification, with typical photographs taken. g, h Representative immunoblots and quantitative analysis of protein level of ICAM-1, VCAM-1, p65, and phosphorylation of p65. (**P < 0.01 C-HDL group compared with control group, ^##P < 0.01, (-)- DHMEQ pretreated group compared with C-HDL group)