Fig. 3

OVA-induced lung histological and tryptase changes in Tespa1^−/− and WT mice as determined by H&E staining, PAS staining, toluidine blue staining, and immunohistochemistry. a Images of H&E staining, PAS staining, toluidine blue staining and immunohistochemistry. b Quantitative analysis of the degree of inflammatory cell infiltration in the lung sections, based on the methods developed by Myou and Lee [45, 46]. c Kruskal–Wallis one-way analysis of mucus production in the lung sections was performed in accordance with the methods developed by Myou and Lee. d Number of mast cells in lung sections. e Immunohistochemical scores of tryptase. KO: Tespa1^−/− mice; WT: wild-type mice. *P < 0.01 between groups. **P < 0.01 between groups. Mean ± SD, n = 8–10 mice per group