Fig. 1

Preparation of organotypic heart slices of mouse LV myocardium. LV tissue was isolated from a transgenic homozygous αMHC-mCherry mouse line and a vibrating microtome was used to obtain sections of 300 µm thickness. Slices cut in parallel to the epicardial surface were punched using a 4 mm-diameter biopsy punch and cultured at the air-liquid interface on cell culture inserts