Fig. 3

OKN-007 and LDN-193189 both decrease apparent diffusion coefficient (ADC) values in a pre-clinical model for DIPG. ADC values were measured from diffusion-weighted imaging (DWI), at the completion of treatment. a The diffusion map for the untreated mouse shows an area of hypointensity/restricted diffusion, likely due to the ventriculomegaly from the tumor burden highlighted with the arrows. This was not seen in mice in either treatment group. b Diffusion map of mice treated with OKN-007. c Diffusion map of mice treated with LDN-193189. d ADC was normalized by subtracting ADC value from the tumor by the ADC value on the contralateral, normal tissue. Untreated mice (closed circles; n = 4; 3 ROIs per sample) had significantly higher ADC values compared to both treatment arms. Although there was no significant difference in ADC between the two treatment arms, both OKN-007 (open squares; n = 4; 3 ROIs per sample) and LDN-193189 (closed triangles; n = 5; 2–3 ROIs per sample) helped normalize the ADC values within the tumor bed. e Normalized ADC values obtained at various treatment time-points. OKN-007 and LDN-193189 had significantly lower normalized ADC values compared to untreated tumors (p < 0.05 and p < 0.01, respectively). An ANOVA (one-way ANOVA, multiple comparisons) statistical test was used