Fig. 1

Example of a CAML, normal white blood cells (WBCs) and circulating tumor cells (CTCs) with size comparisons. Cells were stained with an antibody mixture of CD45 (purple), cytokeratin (green), and DAPI (light blue). a–d A CAML(white open triangle) which was -65 m in length and CD45 + & DAPI + . CAML was attached to a normal WBC − 10 m in length (white arrows) which appears CD45 + and DAPI + . e–h CTCs (white dashed arrows) are Cytokeratin + & CD45−.CTCs shown near to normal WBCs (white arrows) that are CD45 + and DAPI + .Normal WBCs are typically − 8–10 m cells highly expressive of CD45.CAMLs are large, typically CD45 + ,low Cytokeratin + , with polynucleated DAPI nucleus. CTCs are CD45-with high filamented Cytokeratin signal