Fig. 3

Analysis of the interaction of CREB with HLA-G. a In silico analysis of the HLA-G promoter sequence for predicted CREB binding sites. b The protein levels of HLA-G and of CREB were analyzed in RCC cell lines by Western blot. JEG-3 cells served as positive control. c The bar diagram shows the qPCR data expressed as means of three biological replicates of the chromatin immunoprecipitation. In comparison to the unspecific IgG control, the usage of anti-CREB antibody enriched HLA-G promoter regions as well as the internal positive control of the known CREB-bound RRM2 promoter sequence. The lower agarose gel exemplarily shows the enrichment of one HLA-G promoter fragment with the anti-CREB pull down in comparison to the isotype control of the antibody