Fig. 6
Pulsed Mo-DCs cultured in FEP bags or TCPS plates exhibit the same T cell activation capacity. Following Mo-DC differentiation, 15 × 106 T cells were cultured in the presence of unpulsed or CMVpp65 peptide (NLV)-pulsed Mo-DC at 1:10 T cell: Mo-DC ratio. a, b At day 14, the proportion of NVL-specific T cells was assessed by flow cytometry. c, d The activation status of CD8+ T cells from the donor was assessed by the evaluation of CD25 expression. e, f The functionality of CD8+ T cells was evaluated through the expression of cytotoxic degranulation marker CD107a upon restimulation with CMVpp65 (NLV) antigen. g, h Granzyme B expression was evaluated upon re-stimulation with CMVpp65 (NLV) antigen. Panels show representative examples (a, c, e, g) or the mean ± SEM of 4 independent experiments (b, d, f, h). P-values shown are for paired comparisons