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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKKɛ complex inhibitor BX795

Fig. 2

Effect of BX795 treatment on RB-340-1F lentiviral vectors transduction efficiency. a Transgene expression was assessed by flow cytometry on day 6. b T cell transduction rate with the absence of BX795 (BX795-) or presence of BX795 (BX795+). GFP+/mCherry-: GFP single positive population. GFP+/mCherry + : GFP and mCherry double positive population. GFP-/mCherry+ : mCherry single positive population. c MFI of mCherry in CAR-TEV-mCherry transduced cells. d MFI of FITC in LdCK-GFP transduced cells. All results are the averages of four donors, except for figure A, which is flow cytometry plot from a representative donor. *p < 0.05; **p < 0.01

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