Fig. 2

Cohesin binding patterns in the absence of STAG2 highlight STAG1 compensation. a Normalised BigWig binding profiles generated from STAG2, STAG1 and CTCF ChIP-Seq, in STAG2-WT and ΔSTAG2 cells viewed in IGV. Each region shown, represents the typical binding pattern observed in these cells, for each protein throughout the genome and shows the complete loss of functional STAG2, slightly increased binding of STAG1 and slightly decreased binding of CTCF in the ΔSTAG2 cells. b Consensus binding peak lists were generated for STAG2, STAG1 and CTCF from ChIP-Seq carried out in STAG2-WT cells. These lists were then used to plot the genome-wide average binding profiles of STAG2, STAG1 and CTCF at these consensus sites (−2.5–2.5 kb surrounding peak summits) in STAG2-WT and ΔSTAG2 cells. Average binding profiles are normalised to total reads per genome content, centred on peak summits. c Heatmap profiles of binding patterns for STAG2, STAG1 and CTCF across the genome in both STAG2-WT and ΔSTAG2 cells. Binding profiles are plotted from −2–2 Kb either side of the individual binding peak summits for each protein called in the STAG2—WT cells. Colour scale depicts fold binding enrichment at these sites. d Venn diagram of the number of STAG1 binding peaks called in STAG2-WT and ΔSTAG2 cells, with 14,375 peaks present in both STAG2-WT and ΔSTAG2 cells, but significantly more STAG1 binding peaks in ΔSTAG2 cells compared to STAG2-WT cells