MiR-199a-3p/5p participated in TGF-β and EGF induced EMT by targeting DUSP5/MAP3K11 in pterygium

Table 1 The list of primer sequences

Primer name	Forward primer (5′ → 3′)	Reverse primer (5′ → 3′)	Amplicon length (bp)	Tm (°C)
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT	94	60
U6-RT	AACGCTTCACGAATTTGCGT
miR-30a-5p	GCGCTGTAAACATCCTCGAC	GTGCAGGGTCCGAGGTATTC	60	60
miR-30a-5p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTTCCAGT
miR-199-5p	GCCCAGTGTTCAGACTACCTG	GTGCAGGGTCCGAGGTATTC	58	60
miR-199-5p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAACAGGT
miR-143-5p	GGTGCAGTGCTGCATCTCT	GTGCAGGGTCCGAGGTATTC	56	60
miR-143-5p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCA GAGA
miR-486-3p	CGGGGCAGCTCAGTACAG	GTGCAGGGTCCGAGGTATTC	65	60
miR-486-3p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATCCTGTA
miR-199-3p	GCACAGTAGTCTGCACATTGG	GTGCAGGGTCCGAGGTATTC	68	60
miR-199-3p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAACCAAT
miR-675-5p	TATATGGTGCGGAGAGGGC	GTGCAGGGTCCGAGGTATTC	71	60
miR-675-5p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACTGTGG
DUSP5 mRNA	CTACTCGCTTGCCTACCC	ACATCCACGCAACACTCA	95	57
DUSP5 3′UTR	ATACTCGAGAACTGGGATGGAGGAATC	TGGGTCGACTGCTTTTCTCCTTTTACTATTTTTA	1118	53
MAP3K11 mRNA	GTGCCATACCGTGGCATTGA	CATTTCCCGTAGGACCTGTG	204	60
MAP3K11 3′UTR	GCTCGCTAGCCTCGAGGTGGGCCAGGCCACTCCC	ATGCCTGCAGGTCGACTGTGGGGAGACAGCTTTTGAG	536	67
CDH1 (E-cadherin)	GGATTGTCGGATTGGGAGAA	CATTCTGCTGCTTGAGGGTT	194	60
CDH2 (N-cadherin)	GAGTCGAACAGCAGCTCTGA	CTTGTCTGCTTCGGTTTGAC	199	60
VIM (Vimentin)	GATGTTTCCAAGCCTGACCT	CACTTCACAGGTGAGGGACT	216	60
COL1A1	GACTGGGAAACCAGATGCTG	GAAGCCTCTCTCTCCTCTCT	224	60
COL3A1	GAAGGAGGATGTTCCCATCT	GACCATTAGGAGGGCGAGTA	225	60
MMP1	GACTGGGAAACCAGATGCTG	GGTGAATGTCAGAGGTGTGA	226	60

ISSN: 1479-5876