Fig. 2

VLP-delivered HA-Vpr induces a proteasome-dependent decrease in human B lymphocyte uracil removal capacity. a Daudi B-cells were transduced with VLP HA-Vpr wt or mutant (R90K, Q65R or W54R) or ΔVpr at M.O.I of 10. 72 h later cells were lysed and examined by immunoblot for UNG2 content (with anti-UNG2 antibody or anti-α tubulin as control). Lanes were cropped from different parts of a unique gel (see Additional file 2: Supplementary dataset 2). Corresponding UNG activity was measured in the presence or absence of the UNG inhibitor UGI. b Daudi cells were treated for 1 h with 50 µM MG132 and then transduced with the indicated VLPs at MOI of 10. 24 h later, UNG2 content was analyzed with anti-UNG2 by western blotting (lanes were cropped from different parts of a unique gel (see Additional file 2: Supplementary dataset 2)) and UNG activity as in (a). Values are the means of triplicate measurements ± SD. Statistical significance was determined using the ANOVA test. Ut: untransduced