Fig. 2From: Preconditioning with rHMGB1 ameliorates lung ischemia–reperfusion injury by inhibiting alveolar macrophage pyroptosis via the Keap1/Nrf2/HO-1 signaling pathwayrHMGB1 preconditioning decreases I/R-induced AM pyroptosis. a Isolated AM count in BALF. b LDH release from isolated AMs in BALF. c Representative results from flow cytometry to assess macrophage pyroptosis: F4/80+ cells were gated and analyzed for fluorescently labeled active caspase (FLICA) and propidium iodide (PI). d Quantitative analysis of F4/80+FLICA+PI+ cells. e Representative images of isolated AMs in BALF after immunostaining for GSDMD. f GSDMD abundance in isolated AMs. (* p < 0.05, compared to control group, #p < 0.05, comparison between I/R and rHMGB1 + I/R group)Back to article page