Fig. 5
Anti-tumor effects of 32A9 IgG and 32A9 immunotoxin. a Anti-tumor activity of 32A9 IgG in NYG mice with Huh-7 xenograft tumors. Mice are treated with 32A9 IgG (10 mg/kg) or PBS every other day. Arrows indicate antibody injection. Values represent the mean ± SE, with *p < 0.05 and ***p < 0.001. b Schematic diagram of the 32A9 immunotoxin (32A9-mPE24). PE: Pseudomonas exotoxin A. c SDS-PAGE to show the purified 32A9-mPE24. R: reducing. NR: non-reducing. d WST assay to detect the cytotoxicity of 32A9-mPE24. The dashed line indicates the IC50 value. Values represent the mean ± SD