Table 3 Coronavirus 19 spike protein epitop analysis for best MHC class I allele selection on the basis of Highest Consensus percentile rank and Lowest IC50 Value (A & B)
Epitope sl. no. | Peptide sequence (Position within sequence) [Highest Consensus percentile rank (Allele)] A | Peptide sequence (Position within sequence) [Lowest IC50 Value (Allele)] B | Protective Antigen (Threshold = 0.4) C |
|---|---|---|---|
1 | LNEVAKNLNESLIDL [4.10 (HLA-DRB1*13:02)] | LNEVAKNLNESLIDL [59.88 (HLA-DRB1*13:41)] | A & B: 0.3265 (Probable NON-Â ANTIGEN) |
2 | TLVKQLSSNFGAISS [2.90 (HLA-DRB1*04:01)] | VKQLSSNFGAISSVL (963–977) [22.48 (HLA-DRB1*01:13)] | A: 0.3473 (Probable NON-ANTIGEN) B: 0.4397 (Probable ANTIGEN) |
3 | QQLIRAAEIRASANL (1010–1024) [10 (HLA-DRB1*11:01)] | AAEIRASANLAATKM (1015–1029) [11.23 (HLA-DRB1*01:13)] | A: 0.6128 (Probable ANTIGEN) B: 0.7125 (Probable ANTIGEN) |
4 | DKYFKNHTSPDVDLG (1153–1167) [9.90(HLA-DRB1*04:05) | LDKYFKNHTSPDVDL [34.91 (HLA-DRB1*01:13)] | A: 0.6128 (Probable ANTIGEN) B: 0.0787 (Probable NON-ANTIGEN) |
5 | TIMLCCMTSCCSCLK [9.5 (HLA-DRB1*04:04)] | TIMLCCMTSCCSCLK [209.39 (HLA-DRB1*01:13)] | A & B: 0.0231 (Probable NON-ANTIGEN) |
6 | MYICGDSTECSNLLL [9.5 (HLA-DRB1*03:09)] | MYICGDSTECSNLLL [504.59 (HLA-DRB1*03:11)] | A & B: 0.1691 (Probable NON-ANTIGEN) |
7 | GWTFGAGAALQIPFA (885–899) [8.80 (HLA-DRB1*07:03)] | GWTFGAGAALQIPFA (885–899) [14.44 (HLA-DRB1*01:13)] | A & B: 0.4665 (Probable ANTIGEN) |
8 | SIIAYTMSLGAENSV (691–705) [6.60 (HLA-DRB1*04:04)] | IIAYTMSLGAENSVA (692–706) [7.11 (HLA-DRB1*01:13)] | A: 0.5691 (Probable ANTIGEN) B: 0.5426 (Probable ANTIGEN) |
9 | VRDPQTLEILDITPC (576–590) [9.50 (HLA-DRB1*04:08)] | VRDPQTLEILDITPC (576–590) [536.29 (HLA-DRB1*14:31)] | A & B: 1.1285 (Probable ANTIGEN) |
10 | VSVITPGTNTSNQVA [7.90 (HLA-DRB1*04:08)] | ITPGTNTSNQVAVLY (598–612) [326.79 (HLA-DRB3*03:01)] | A: 0.3887 (Probable NON-ANTIGEN) B: 0.7193 (Probable ANTIGEN) |