Fig. 4

The Notch pathway is activated during the MSC-induced differentiation of mDCs into DCregs. a, b The expression of Notch1, Notch2 and Notch3 protein levels in DCs that were cultured with or without MSCs for 72 h was evaluated using Western blot analysis. c, d The percentage of Notch2-positive cells in CD11c⁺ DCs that were cultured with or without MSCs for 72 h was detected by flow cytometry. e, f The expression of Jagged1 and Jagged2 protein levels in MSCs that were cultured with or without LPS for 24 h was evaluated using Western blot analysis. g, h The percentage of Notch2-positive cells in CD11c⁺ DCs that were cultured with or without rhjagged1 for 72 h was detected by flow cytometry. n = 3, b, d and h *p < 0.05 versus mDC; f *p < 0.05 versus MSC; the data are expressed as the mean ± SD