Fig. 5

miR-128 regulates NF-KB/p-JNK axis through CB1 to affect the occurrence of diabetic bladder disease. a STRING website analysis of gene interaction network diagram; b–d Western blotting detection of NF-KB and p-JNK and apoptosis in bladder tissue of three groups of NC, PU and DM rats Expression of related protein Bcl2, * indicates compared with NC group P < 0.05; e Western blotting detected NF-KB in rat bladder tissue of mimic NC group, miR-128 mimic group, inhibitor NC group and miR-128 inhibitor group, P-JNK and apoptosis-related protein Bcl2 expression, * indicates compared with mimic NC group P < 0.05, # indicates compared with inhibitor NC group P < 0.05; f Expression of NF-KB, p-JNK and apoptosis-related protein Bcl2 in bladder tissue of rats in each group by Western blotting, * indicates compared with the mimic NC + oe-NC group P < 0.05, # indicates compared with the miR-128 mimic + oe-NC group P < 0.05. g qPCR was used to detect the expression level of miR-128 in bladder tissue of rats in each group, * indicates compared with mimic NC + DMSO group P < 0.05; h qPCR detection of CB1 expression in bladder tissues of rats in each group, * indicates compared with mimic NC + DMSO group P < 0.05; i Western blotting detection of CB1, NF-KB, p-JNK and apoptosis-related protein Bcl2 in rat bladder tissue of each group Expression, * indicates compared with the mimic NC + DMSO group P < 0.05, # indicates compared with the miR-128 mimic + DMSO group P < 0.05; j statistical map of blood glucose levels in rats; k statistical map of body weights in rats; l 24 h urine volume statistics of rats; m bladder volume statistics of rats; n Statistics chart of average residual urine volume in rats; o Statistics chart of single urine output of rats; p Statistics chart of average urine output of rats; r Rat bladder weight statistics; s Bladder tissue section HE staining (× 100). Figure j–r, * indicates the comparison with mimic NC + DMSO group P < 0.05, # indicates the comparison with miR-128 mimic + DMSO group P < 0.05. The above results are all measurement data, expressed as mean ± standard deviation. One-way ANOVA and Tukey’s post hoc test was used to compare data between multiple groups