Fig. 3

miR-128 targets CB1 expression. a Venn diagram of bioinformation website microT, TargetScan, miRWalk and RNAInter predicting the intersection of downstream target genes of miR-128; b STRING website analyzes the interaction relationship network diagram of 51 candidate genes, circled in the figure Degree from large to small indicates the Degree of the gene, circle color from blue to orange indicates Degree from large to small, and the line in the middle of the circle indicates the co-expression relationship between genes; c Biology website predicts the binding position of miR-128 and CB1 Points; d Fluorescent activity detection results of miR-128 and CB1, * indicates comparison with the mimic NC group P < 0.05; e RIP experiments detect the binding of miR-128 and CB1 with Ago2, * indicates comparison with the anti-IgG group P < 0.05; f RT-qPCR detects the relative expression level of CB1 in bladder tissue, * indicates compared with NC group P < 0.05, # indicates compared with PU group P < 0.05; g miR-128 and CB1 correlation analysis; h RT-qPCR detection of each group Relative expression level of CB1 in rat bladder tissue; i Western blotting detection of CB1 protein expression level in rat bladder tissue in each group; j CB1 protein expression level statistical map. (h–j) * indicates that compared with the mimic NC group P < 0.05, # indicates that compared with the inhibitor NC group P < 0.05 The above results are measured data, expressed as mean ± standard deviation. Paired T test. One-way ANOVA was used to compare data between multiple groups. Tukey’s was used for post hoc tests