Table 1 List of reagents used for cell culture
From: Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes
Materials | Abbreviations | Company | Catalogue# |
|---|---|---|---|
Bone morphogenetic proteins 4 | BMP4 | Peprotech | 120-05 |
Vascular endothelial growth factor | VEGF | Peprotech | 100-20 |
Wnt3A | Â | Peprotech | 315-20 |
Activin A | Â | Peprotech | 120-14E |
GSK-3β Inhibitor VIII |  | Calbiochem | 361549 |
Fibroblast growth factor alpha | FGFa | Peprotech | 100-17A |
β-Estradiol |  | Sigma-Aldrich | E2257 |
Insulin-like growth factor 2 | IGF2 | Peprotech | 100-12 |
Thrombopoietin | TPO | Peprotech | 300-18 |
Heparin | Â | StemCell Technologies | 07980 |
3-Isobutyl-1-methylxanthine | IBMX | Sigma-Aldrich | I5879 |
StemRegenin 1 | SR1 | Cellagen Technology | C7710 |
Hydrocortisone | HC | Sigma-Aldrich | H0888 |
Stem cell factor | SCF | Peprotech | 300-07 |
Interleukin 3 | IL-3 | Peprotech | 200-03 |
Erythropoietin | EPO | Stem Cell | 02625 |
Poloxamer 188 | P188 | Sigma-Aldrich | P5556 |
Transferrin | TF | Sigma-Aldrich | T8158 |
Insulin | Â | Sigma-Aldrich | I3536 |
Ferric nitrate | FN | Sigma-Aldrich | F8508 |
Monothioglycerol | MTG | Sigma-Aldrich | M6145 |
Penicillin–Streptomycin | P-S | Gibco | 15140122 |
Y-27632 | Â | StemCell Technologies | 72392 |
Stemline II hematopoietic stem cell expansion medium | Â | Sigma-Aldrich | S0192 |
mTeSR1 basal medium | Â | StemCell technologies | 85851 |
mTeSR1 5 × Supplement |  | StemCell technologies | 85852 |
AggreWell EB formation medium | Â | StemCell technologies | 05893 |