Fig. 5

FTY720 suppressed accumulation of collagen in tissue of GvHD mice and reduced fibrosis marker protein in human skin fibroblast. a Immunofluorescence staining of skin, large and small intestine after BMT; the micrographs are from 1 of 2 independent experiments. The sections in paraffin molds were stained with a Picro Sirius Red Stain(original magnification ×200). Tissues were collected on day 28 after transplantation. b Proteins in lysates from human skin fibroblast treated with 10 ng/ml TGF-β1 were separated and subjected to Western blotting using the αSMA, CTGF, and Fibronectin antibodies