Fig. 4

Rapid ubiquitination of WT1 protein by WP1130. a The protein expression of WT1 was measured in THP1 and Kasumi-1 cells treated with 5.0 μM WP1130 in the presence or absence of 5.0 μM MG132 for 24 h. b Ubiquitin was assessed in THP1 and Kasumi-1 cells treated with 2.5 and 5.0 μM WP1130 for 4 h. c THP1 cells were treated with 5.0 μM MG132 in the presence or absence of 5 μM WP1130 for 24 h, followed by co-immunoprecipitation with anti-WT1 antibody and immunoblotted for ubiquitin. The Anti-IgG antibody was used for negative control