Fig. 2

Comparison of differentiation status of CD8⁺dT cells and CD8⁺pT cells. The gating strategy of CD8⁺T cells from maternal peripheral blood (a) and decidua (b). The memory phenotype was evaluated by expression of CD45RA and CCR7, and each of the CCR7 versus CD45RA dot plot quadrants were further analyzed for CD27 versus CD28 staining. c Cell surface markers CD45RA and CCR7 were used to classify naive (CD45RA⁺CCR7⁺), terminally differentiated effector memory (EFF or TEMRA; CD45RA⁺/CCR7⁻), effector-memory (EM; CD45RA⁻CCR7⁻), and central-memory (CM; CD45RA⁻CCR7⁺) CD8⁺ T cells. d The proportion of each subset of CD8⁺dT cells and CD8⁺pT cells. n = 15, paired t⁻test, *p = < 0.05, **p = < 0.01, ***p = <0.005, ****p = <0.0001