Fig. 2

Sensitivity differences between the U-assay and 2-LTR assay for unintegrated HIV DNA detection. a Quantification of unintegrated HIV DNA and 2-LTR circles in blood samples of Off-ART patients (n = 12), viremic (n = 14) and aviremic (n = 29) On-ART patients, and untreated infected controller patients (HIC, n = 20). The dotted line indicates the limit of quantification (QL, 2 copies). Detectable values below the QL are depicted by empty symbols. b Proportion of samples from the four groups of HIV-1 patients, with quantifiable (colored bars) or detectable (white bars) levels of unintegrated HIV DNA (black) and 2-LTR circles (gray). The number of samples from each group quantified or detected below the QL by each assay is indicated. The frequency with which the levels of unintegrated viral DNA were quantified was higher using the U-assay than the 2-LTR assay (overall p < 0.0001, Fisher’s exact test)