Fig. 2

Graphene oxide inhibits the proliferation and survival of GSCs. a, b EdU staining indicated the cell proliferation capability of GSCs treated with 50 μg/ml GO for 2 days or that were untreated. The right panel shows the quantification of EdU-positive cells. Scale bar = 100 μm. c MTT assay indicated the cell viability of GSCs with or without treatment with different dosages of GO for 2, 4, and 6 days. d, e TUNEL staining of GSCs showed an increase in cell apoptosis after treatment with 50 μg/ml GO for 2 days. The right panel shows the quantification of the TUNEL-positive cells. Scale bar = 100 μm. *p < 0.05, **p < 0.01. Data represent the mean ± SEM of at least three independent experiments