Fig. 5

Elevated expression of miR-1271 suppresses the progression of prostate cancer both in vivo and in vitro. a miR-1271 expression and PES1 mRNA expression in PC-3 cells detected by RT-qPCR. b Images of EdU assay (200×), positive rate of PC-3 cells as well as the expression of Ki67 and PCNA, following treatment of NC mimic, miR-1271 mimic or their respective NC detected by EdU assay and Western blot analysis. c PC-3 cell apoptosis and protein expression of c-caspase-3/caspase-3 and c-caspase-9/caspase-9 in PC-3 cells following treatment of NC mimic, miR-1271 mimic or their respective NC tested by flow cytometry and Western blot analysis. d Images (200 ×) and quantitative analysis of PC-3 cell migration and invasion and protein expression of MMP-2 and MMP-9 in PC-3 cells following treatment of NC mimic, miR-1271 mimic or their respective NC evaluated by Transwell assay and Western blot analysis. e miR-1271 expression and PES1 mRNA expression in mouse tumors detected by RT-qPCR. f Representative tumors and tumor growth rate of mice bearing human prostate cancer cell xenografts in response to NC agomir, miR-1271 antagomir or their respective NC (n = 6 for each group). *p < 0.05, vs. treatment with NC mimic or NC agomir. #p < 0.05, vs. treatment with NC inhibitor or NC antagomir. Data obtained from three independent experiments were presented as mean ± standard deviation and analyzed using independent sample t-test between two groups and using one-way ANOVA among multiple groups. Tumor size over indicated time points were compared using two-way ANOVA