Fig. 2

Silencing of PES1 inhibits the development of prostate cancer in vivo and in vitro. a PES1 mRNA expression in PC-3 cells detected by RT-qPCR. b Images of EdU assay (200×), positive rate of prostate cancer PC-3 cells and the expression of Ki67 and PCNA in response to si-NC or si-PES1 detected by EdU assay and Western blot analysis. c PC-3 cell apoptosis and protein expression of c-caspase-3/caspase-3 and c-caspase-9/caspase-9 in response to si-NC or si-PES1, as measured using flow cytometry and Western blot analysis. d PC-3 cell migration and invasion (200×) and protein expression of MMP-2 and MMP-9 in response to si-NC or si-PES1, as measured using Transwell assay and Western blot analysis. e PES1 mRNA expression in mouse tumors detected by RT-qPCR; f representative tumors and tumor growth rate of mice bearing human prostate cancer cell xenografts in response to sh-NC or sh-PES1 treatment (n = 6 for each group). *p < 0.05, vs. treatment with si-NC or sh-NC. Data obtained from three independent experiments were presented as mean ± standard deviation and analyzed using independent sample t-test between two groups and using one-way ANOVA among multiple groups. Tumor size across indicated time points was compared using two-way ANOVA