Fig. 6

Silencing of HSP70 blocked the inhibition of 17AAG on sevoflurane-induced upregulation of IL-6 and activation of NF-κB signaling pathway. H4 cells were transfected with vehicle, or siRNA against human HSPA1A (HSPA1A siRNA), or non-targeting siRNA (control siRNA), and received treatments as in Fig. 2. The expression of (a) HSP70, (b) IL-6, (c) p-IκBα, (d) IκBα, (e) cytoplasmic NF-κB and (f) nucleus NF-κB proteins were analyzed by Western blot. β-actin and Histone H3 were used as loading controls for cytoplasmic and nucleus extracts, respectively. Shown representative blots of 3 independent experiments. n = 6. Mean ± SD, **P < 0.01 vs. SEV, ## P < 0.01 vs. SEV + 17AAG by one-way ANOVA followed by Bonferroni multiple comparisons test