Fig. 3

17AAG inhibited sevoflurane-induced upregulation of IL-6 and activation of NF-κB signaling pathway. H4 cells were treated as in Fig. 2. a, b The expression of IL-6 and proteins of the NF-κB signaling pathway (p-IκBα, IκBα, cytosolic NF-κB p65 and nucleus NF-κB p65) were examined by Western blot using whole or fractionated H4 cell lysate. Histone H3 and β-actin were used as loading controls for nucleus and cytoplasmic extracts, respectively. c Immunofluorescence staining of NF-κB p65 distribution in treated H4 cells (×400 magnification; scale bar, 20 μm). Representative images from 3 independent experiments. n = 6 (a, b). Mean ± SD, **P < 0.01 vs. CON, ## P < 0.01 vs. SEV by one-way ANOVA followed by Bonferroni multiple comparisons test