Fig. 4

In vitro drug sensitivity test using low attached 3D cell culture system. a MSE samples were treated with carboplatin (50 µM), pemetrexed (5 µM) and pembrolizumab (2.5 nM) in a single or combinatorial manner, N = 4. Cell proliferation readout of each group was shown as value ± SD compared with the corresponding vehicle group (control group). *p-value < 0.05 other groups compared with the control group; ^#p-value < 0.05 combinatorial test groups compared with Carbo and Peme test groups; ^∆p-value < 0.05 C + P + Pembro triple test group compared with C + P combinatorial test group. b PD-L1, CD3 and diagnostic (Dx) marker staining of original cell blocks, scale bar = 100 µm. Arrow: tumour cell. Abbreviations: carboplatin (carbo, C), pemetrexed (peme, P), pembrolizumab (pembro)