Fig. 4

Changes in H4 acetylation pattern distributed among epithelial compartments. a Day 1 after wounding depicts the global deacetylation of histone H4 lysines at the adjacent and normal epithelia. Normal and adjacent epidermis display similar patterns (n = 8 mice). b H4K12 is hyperacetylated by day 4 after injury at the adjacent epithelium and epithelial tongue, compared to the normal epithelium (n = 8 mice). c On day 9, we observed the complete closure of the epidermis. H4K12 is shown hyperacetylated at the normal epidermis, and H4K16 is hyperacetylated at the closed wound site. Note that overall acetylation levels of H4K12 are higher than other acetylation sites of histone H4 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) (n = 6 mice; data represented as mean ± SEM)