Fig. 8

FOXO4 overexpression abrogates the miR-328-3p-mediated cell injury. THLE-2 cells were co-transfected with pHBV1.3 or empty pcDNA3.1 vector (control), and miR-328-3p mimic or mimic negative control (NC), under LPS (1 μg/mL, 24 h) and HBV stimulation. a Cell proliferation at different time points was examined by MTT assay. b Cell apoptosis was examined by flow cytometry and the apoptosis rate was shown. c Levels of TNF-α, IL-6, IL-8, IL-12, and IL-18 were measured by ELISA. Values are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. LPS + HBV + Vector + mimic NC. ^#P < 0.05, ^##P < 0.01 vs. LPS + HBV + Vector + miR-328-3p mimic