Fig. 1

Gating strategy for the monocyte subsets and TLR expression assessment. Classical monocytes were defined as CD14++, CD16−; intermediate monocytes as CD14++, CD16+ and non-classical monocytes as CD14+, CD16++. The expression of TLRs was assessed by measuring the mean fluorescence intensity (MFI) of TLR2 and TLR4 in every monocyte subset