Fig. 5

NZ9000SHD-5 inhibited the secretion of pro-inflammatory cytokines in LPS-induced RAW.264.7 cells. RAW264.7 cells were incubated with LPS (1 ug/ml) for 12 h. Expression of a IL-6, b TNF-α, c IL-1βmRNA was assessed with real-time PCR. d–f The levels of inflammatory cytokines in the supernatant were assessed by ELISA. Differences between groups were determined by ANOVA followed by the post hoc LSD test (n = 4). *p < 0.05 as compared to the DSS group. ^#p < 0.05 as compared to the NZ9000 group