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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: An engineering probiotic producing defensin-5 ameliorating dextran sodium sulfate-induced mice colitis via Inhibiting NF-kB pathway

Fig. 1

Construction of recombinant Lactococcus lactis. a The constructing procedure of NZ9000SHD-5. b The defensin gene (115 bp) was confirmed to be successfully ligated to the secreting plasmid pNZ8148-sp. c Gene sequencing of recombinant NZ9000SHD-5 confirmed the successful construction of the recombinant Lactococcus lactis. d The expression of defensin in the supernatant was measured by Western Blotting with specific antibodies. (Bar 1: recombinant NZ9000SHD-5 with nisin; Bar 2: recombinant NZ9000SHD-5 without nisin; Bar 3: recombinant NZ9000 with nisin)

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Journal of Translational Medicine

ISSN: 1479-5876

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