Fig. 5

Effects of overexpression of ABAT and ALDH6A1 on ccRCC cell growth. a ACHN cells stably transfected with ABAT or ALDH6A1 lentivirus and performed by Western blot analysis. b The growth inhibition rates were measured by WST-1 assay kit at 24, 48, and 72 h in ACHN cells that stably expressed ABAT or ALDH6A1. c Representative images of clonogenic assays of ACHN cells that stably expressed ABAT or ALDH6A1. d ACHN cells stably expressed ABAT or ALDH6A1. Cell death was detected using an ELISA-based cell death detection kit. e Western blot assay was performed in ABAT or ALDH6A1 overexpressed 786-O cells. f WST-1 assay in 786-O cells that stably expressed ABAT or ALDH6A1. g Colony formation assay in 786-O cells that stably expressed ABAT or ALDH6A1. h Cell death assay in 786-O cells that stably expressed ABAT or ALDH6A1. i Representative images of migration assays of ACHN cells stably expressing ABAT or ALDH6A1 (left) and quantification of the relative migration cell number (right). Scale bar, 100 µm. All experiments were repeated three times. The p value was measured using Student’s t-test; *p < 0.05, compared with the control cells. Ctrl: cells transfected with empty control lentivirus