Fig. 2

Specific binding between lncARSR and YAP1 inhibits YAP1 phosphorylation and accelerates YAP1 nuclear translocation. a Binding relationship between lncARSR and YAP1 detected by RNA pull down. b Interaction between lncARSR and YAP1 further verified by RIP assay. c Co-localization of lncARSR and YAP1 examined by RNA FISH (×400). d Levels of YAP1 and phosphorylated YAP1 in liver of NAFLD mice and normal mice determined by western blot analysis (n = 15). e Levels of YAP1 and phosphorylated YAP1 in nucleus and cytoplasm of HepG2 cells treated with oleate monitored by western blot analysis. f Co-localization between lncARSR and YAP1 in cells treated with oleate examined by RNA FISH (×400). All the measurement data were expressed as mean ± standard deviation. Comparisons among multiple groups were analyzed with one-way ANOVA, and the unpaired t-test was made for analysis between two groups. The experiment was repeated three times. *p < 0.05 against normal diet-fed mice or control HepG2 cells