Fig. 11

Effect of adjuvants on ex vivo differentiation of naïve CD4⁺ T cells from cancer patients and healthy subjects. Isolated PBMCs were incubated with adjuvants and phenotype of CD4⁺ T cells was assessed by flow cytometry. Naïve (TN) CD4⁺ T were identified as CD4⁺/CD45RA⁺/CD62L⁺; Effector cells (TE) as CD4⁺/CD45RA⁺/CD62L⁻; Central Memory (TCM) as CD4⁺/CD45RO⁺/CD62L⁺; Effector Memory (TEM) as CD4⁺/CD45RO⁺/CD62L⁻. In the panel of Naïve cells, the samples treated with RNAdjuvant are significantly lower compared to samples treated with PBS in Healthy, OT-pre and OT-post subjects (p < 0.01)