Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation

Table 1 Lymphoid subpopulations detected in whole blood, and PBMCs from automatic and manual processing

Sample ID	Sample processing	T-cells (CD3+/CD56−) (%)	B-cells (CD19+/CD3−) (%)	NK-cells (CD3−/CD56+) (%)	NKT (CD3+/CD56+) (%)
1	Wb	57	3	27	9
	Automatic	56	6	27	8
	Manual	52	9	27	8
2	Wb	82	8	5	2
	Tecan	76	14	7	2
	Manual	77	13	7	2
3	Wb	80	4	5	9
	Automatic	69	9	8	13
	Manual	71	10	7	10
4	Wb	61	2	20	7
	Automatic	57	4	29	4
	Manual	58	3	34	3
5	Wb	63	8	15	5
	Automatic	52	11	24	9
	Manual	44	13	30	6
6	Wb	62	9	18	3
	Automatic	65	10	18	3
	Manual	66	7	17	3
7	Wb	41	18	21	17
	Automatic	44	16	24	11
	Manual	44	15	21	13
8	Wb	68	11	7	13
	Automatic	63	18	6	11
	Manual	60	19	7	12
9	Wb	55	8	25	7
	Tecan	50	8	27	7
	Manual	50	8	28	6
10	Wb	83	5	6	4
	Automatic	74	23	1	1
	Manual	65	27	2	2
11	Wb	59	16	17	5
	Automatic	55	22	19	3
	Manual	55	22	19	4
12	Wb	57	0	28	2
	Automatic	54	0	36	2
	Manual	55	0	33	1
13	Wb	56	9	5	23
	Automatic	55	17	6	18
	Manual	53	18	5	19
14	Wb	68	2	12	14
	Automatic	61	10	10	11
	Manual	56	7	19	11
15	Wb	61	1	8	26
	Automatic	57	3	10	27
	Manual	62	3	8	25
16	Wb	75	4	8	9
	Automatic	70	9	9	9
	Manual	63	14	10	8
17	Wb	59	7	20	7
	Automatic	56	9	21	4
	Manual	58	9	20	4
18	Wb	61	3	27	3
	Automatic	64	7	21	3
	Manual	65	8	21	4
19	Wb	67	3	13	13
	Automatic	59	13	11	13
	Manual	57	10	12	16

ISSN: 1479-5876