Fig. 7

Determination of lymphoid subsets obtained by whole blood, manual and automatic processing. Multicolor flow cytometry analysis shows T, B, NK, and NKT cell percentages in whole blood samples (a) and PBMCs derived from the automatic (b) and manual (c) methods. Left columns show the lymphocyte gating strategy in a CD45 vs SSC dot plot. Middle columns show T, NK and NKT lymphocyte percentages in a CD56 vs CD3 dot plot in terms of CD3^posCD56^neg (green events), CD3^negCD56^pos (purple events) and CD3^posCD56^pos (blue events), respectively. The B lymphocyte percentage was derived as CD19^posCD3^neg events in the CD19 vs CD3 dot plot (right columns). On the right, the line charts show the before-after graphs to compare the percentage of each lymphocyte subpopulation in whole blood and in PBMCs after automatic and manual separation (d)