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Table 4 Summary of publications evaluating antigenicity quality

From: In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

Analytical techniqueEvaluated parameterPre-analytical factorThresholdOrganReferences
Quantitative IF (AQUA score)ER, HER2, Ki-67, CKStorage timeIF signal decreases 10% in 4–8 years depending on the markerBreast[65]
Increased marker: 95th percentile of slope for n = M is higher than 0CITLabile and loss of antigenicity within 1–2 h of CITBreast[66]
Decreased marker: 95th percentile of slope for n = M is lower than 0
No changes in marker: 95% CI for the slope with both n = M and n = 10 × M Including the zero slope
Trend up/trend down: 95% CI for the slope with n = 10 × M not including it
Cytokeratin, pERK1/2 and pHSP-27 expressionCITNegative TQI values (as indicator of loss of tissue quality) for increasing CITBreast[67]
IHCVimentinFixationMelanoma[68]
ER and PgRFixation, slicing, storage of slidesSamples for ER and PgR testing are fixed in 10% NBF for 6 to 72 h. CIT < 1 h. Samples should be sliced at 5-mm intervals. Storage of slides for more than 6 weeks before analysis is not recommendedBreast[69]
P-p27[70]
SNRPA and SnRNP70 H-scoreFixationH-score < 60 as a cut off for positive signalBreast[71]
MAP2Fixation, slicing and storageDecrease of MAP2 immunoreactivity in unfixed and in delayed-fixedRat brain[72]
Actin, desmin and progesterone receptor stainingStorage timeConsistent staining over 18 yearsMesenchyme[63]