Skip to main content

Table 4 The processing and storage of biomaterials in the OncoLifeS data-biobank

From: The OncoLifeS data-biobank for oncology: a comprehensive repository of clinical data, biological samples, and the patient’s perspective

Serum10 mL serum clot tube (BD 367896)Centrifuge (10 min, 1300g, RT)—aliquot serum 5×, store at − 80 °C
EDTA-plasma + buffy coat (DNA)10 mL K2-EDTA tube (BD 367525)Centrifuge (10 min, 1300g, RT), aliquot buffy coat 1×, aliquot EDTA-plasma 5× store at − 80 °C
Heparin-plasma10 mL Li-heparin tube (BD 367526)
2 × 10 mL Li-heparin tube (BD 367526)
Centrifuge (10 min, 1300g, RT), aliquot heparin-plasma 5× store at − 80 °C
Monoculear cell isolation over lymphoprep, 100e6 MNCs per vial, stored in liquid nitrogen
Plasma (for cell-free DNA)2 × 8 mL tube (Streck 218997)Centrifuge (20 min, 1600g, RT), collect plasma, centrifuge (10 min, 16,000g, RT), aliquot cell-free DNA plasma 10×, store at − 80 °C
Whole blood (for DNA)10 mL K2-EDTA tube (BD 367525)No processing, store tube at − 80 °C
Whole blood (for RNA)Paxgene tube (BD 762165)No processing, store tube at − 80 °C
Bone marrow3 × 10 mL syringe with Na-heparinIsolation of mononuclear cells by density centrifugation, controlled freezing of isolated mononuclear cells, store in liquid nitrogen
Feces swab in glycerol bGlycerol swab (Copan Custom kit M1016004)No processing, store tube with swab at − 80 °C
Feces3 × 2.0 mL cryotubes (Greiner 122280)No processing, store cryotubes at − 80 °C
Urine9.5 mL urine tube (BD 365000)Centrifuge (10 min, 1300g RT), aliquot urine 6×, store at − 80 °C
Tumor tissue (for paraffin embedding)Tissue fixed in formalinTissue embedded in paraffin, store at RT
Tissue adjacent to a tumor (for frozen section)Tissue in tinTissue in tin, store at − 80 °C
Tissue adjacent to a tumor (for paraffin embedding)Tissue fixed in formalinTissue embedded in paraffin, store at RT
Healthy tissue (for frozen section)Tissue in tinTissue in tin, store at − 80 °C
  1. RT room temperature