Table 4 The processing and storage of biomaterials in the OncoLifeS data-biobank

Serum

10 mL serum clot tube (BD 367896)

Centrifuge (10 min, 1300g, RT)—aliquot serum 5×, store at − 80 °C

EDTA-plasma + buffy coat (DNA)

10 mL K2-EDTA tube (BD 367525)

Centrifuge (10 min, 1300g, RT), aliquot buffy coat 1×, aliquot EDTA-plasma 5× store at − 80 °C

Heparin-plasma

10 mL Li-heparin tube (BD 367526)

2 × 10 mL Li-heparin tube (BD 367526)

Centrifuge (10 min, 1300g, RT), aliquot heparin-plasma 5× store at − 80 °C

Monoculear cell isolation over lymphoprep, 100e6 MNCs per vial, stored in liquid nitrogen

Plasma (for cell-free DNA)

2 × 8 mL tube (Streck 218997)

Centrifuge (20 min, 1600g, RT), collect plasma, centrifuge (10 min, 16,000g, RT), aliquot cell-free DNA plasma 10×, store at − 80 °C

Whole blood (for DNA)

10 mL K2-EDTA tube (BD 367525)

No processing, store tube at − 80 °C

Whole blood (for RNA)

Paxgene tube (BD 762165)

No processing, store tube at − 80 °C

Bone marrow

3 × 10 mL syringe with Na-heparin

Isolation of mononuclear cells by density centrifugation, controlled freezing of isolated mononuclear cells, store in liquid nitrogen

Feces swab in glycerol b

Glycerol swab (Copan Custom kit M1016004)

No processing, store tube with swab at − 80 °C

Feces

3 × 2.0 mL cryotubes (Greiner 122280)

No processing, store cryotubes at − 80 °C

Urine

9.5 mL urine tube (BD 365000)

Centrifuge (10 min, 1300g RT), aliquot urine 6×, store at − 80 °C

Tumor tissue (for paraffin embedding)

Tissue fixed in formalin

Tissue embedded in paraffin, store at RT

Tissue adjacent to a tumor (for frozen section)

Tissue in tin

Tissue in tin, store at − 80 °C

Tissue adjacent to a tumor (for paraffin embedding)

Tissue fixed in formalin

Tissue embedded in paraffin, store at RT

Healthy tissue (for frozen section)

Tissue in tin

Tissue in tin, store at − 80 °C