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Table 5 Digestion, Mutation, toxicity, allergenicity, hydro and physiochemical profiling of selected peptides

From: Epitope‐based peptide vaccine design and target site depiction against Middle East Respiratory Syndrome Coronavirus: an immune-informatics study

Sr# Peptides Non-digesting enzymes Mutation position Toxicity Allergenicity Hydrophobicity Hydrophilicity Charge PI Mol. weight
MHC class-I binding peptides
1 YKLQPLTFL Trypsin R, Clostripain, IodosoBenzoate, AspN, Cyanogen Bromide, Staph Protease NM NT NA 0.07 − 0.92 1.00 8.59 1122.37
2 LTLLEPVSI Trypsin, Clostripain, AspN, Chymotrypsin, Cyanogen Bromide, IodosoBenzoate, Trypsin R, Trypsin K NM NT NA 0.25 1.80 − 1.00 4.00 984.20
3 ESAALSAQL Clostripain, Chymotrypsin, Cyanogen Bromide, AspN
Trypsin K, IodosoBenzoate, Proline Endopept, Trypsin R
NM NT NA 0.05 − 0.31 − 1.00 4.00 888.97
4 IAGLVALAL Trypsin, Staph Protease, AspN, Chymotrypsin, Trypsin R, Clostripain, Trypsin K, CyanogenBromide, IodosoBenzoate, Proline Endopept NM NT NA 0.43 − 1.20 0.00 5.52 840.72
5 AGYKVLPPL Clostripain, IodosoBenzoate, Staph Protease, AspN, Trypsin R, Cyanogen Bromide NM NT NA 0.13 − 0.67 1.00 8.63 957.18
6 WPRPIDVSK Trypsin, Chymotrypsin (modified)Chymotrypsin, Cyanogen Bromide, Trypsin K, Trypsin R, Staph Protease NM NT NA − 0.26 0.29 1.00 8.75 1097.28
MHC class-II binding peptides
1 YCILEPRSG Cyanogen Bromide, Trypsin K, AspN, IodosoBenzoate NM NT A − 0.11 − 0.06 0.00 5.99 1037.20
2 LYFMHVGYY Trypsin, AspN, Clostripain, IodosoBenzoate, Staph Protease, Trypsin K, Trypsin R, Proline Endopept NM NT NA 0.20 − 1.61 0.50 6.74 1192.40
3 MRLASIAFN IodosoBenzoate, Trypsin K, AspN, Staph Protease, Proline Endopept NM NT A − 0.07 − 0.47 1.00 9.50 1022.23
4 FGITQTAQG Trypsin, AspN, Clostripain, Cyanogen Bromide, IodosoBenzoate, Trypsin R, Proline Endopept, Staph Protease, Trypsin K NM NT NA 0.02 − 0.58 0.00 5.52 922.01
5 VRIGAAANS Chymotrypsin, Trypsin K, IodosoBenzoate, Proline Endopept, Staph Protease, Cyanogen Bromide, AspN M NT A − 0.07 − 0.10 1.00 9.72 857.97
6 VYKLQPLTF AspN, Trypsin R, Clostripain, Staph Protease, Cyanogen Bromide, IodosoBenzoate NM NT NA 0.02 − 0.79 1.00 8.56 1108.35
  1. NM no mutation, NT non-toxic, T toxic, NA non-allergic, A allergic and the non-digesting enzymes showing those enzymes which do not digest peptides into fragments