Table 5 Digestion, Mutation, toxicity, allergenicity, hydro and physiochemical profiling of selected peptides
Sr# | Peptides | Non-digesting enzymes | Mutation position | Toxicity | Allergenicity | Hydrophobicity | Hydrophilicity | Charge | PI | Mol. weight |
|---|---|---|---|---|---|---|---|---|---|---|
MHC class-I binding peptides | ||||||||||
1 | YKLQPLTFL | Trypsin R, Clostripain, IodosoBenzoate, AspN, Cyanogen Bromide, Staph Protease | NM | NT | NA | 0.07 | − 0.92 | 1.00 | 8.59 | 1122.37 |
2 | LTLLEPVSI | Trypsin, Clostripain, AspN, Chymotrypsin, Cyanogen Bromide, IodosoBenzoate, Trypsin R, Trypsin K | NM | NT | NA | 0.25 | 1.80 | − 1.00 | 4.00 | 984.20 |
3 | ESAALSAQL | Clostripain, Chymotrypsin, Cyanogen Bromide, AspN Trypsin K, IodosoBenzoate, Proline Endopept, Trypsin R | NM | NT | NA | 0.05 | − 0.31 | − 1.00 | 4.00 | 888.97 |
4 | IAGLVALAL | Trypsin, Staph Protease, AspN, Chymotrypsin, Trypsin R, Clostripain, Trypsin K, CyanogenBromide, IodosoBenzoate, Proline Endopept | NM | NT | NA | 0.43 | − 1.20 | 0.00 | 5.52 | 840.72 |
5 | AGYKVLPPL | Clostripain, IodosoBenzoate, Staph Protease, AspN, Trypsin R, Cyanogen Bromide | NM | NT | NA | 0.13 | − 0.67 | 1.00 | 8.63 | 957.18 |
6 | WPRPIDVSK | Trypsin, Chymotrypsin (modified)Chymotrypsin, Cyanogen Bromide, Trypsin K, Trypsin R, Staph Protease | NM | NT | NA | − 0.26 | 0.29 | 1.00 | 8.75 | 1097.28 |
MHC class-II binding peptides | ||||||||||
1 | YCILEPRSG | Cyanogen Bromide, Trypsin K, AspN, IodosoBenzoate | NM | NT | A | − 0.11 | − 0.06 | 0.00 | 5.99 | 1037.20 |
2 | LYFMHVGYY | Trypsin, AspN, Clostripain, IodosoBenzoate, Staph Protease, Trypsin K, Trypsin R, Proline Endopept | NM | NT | NA | 0.20 | − 1.61 | 0.50 | 6.74 | 1192.40 |
3 | MRLASIAFN | IodosoBenzoate, Trypsin K, AspN, Staph Protease, Proline Endopept | NM | NT | A | − 0.07 | − 0.47 | 1.00 | 9.50 | 1022.23 |
4 | FGITQTAQG | Trypsin, AspN, Clostripain, Cyanogen Bromide, IodosoBenzoate, Trypsin R, Proline Endopept, Staph Protease, Trypsin K | NM | NT | NA | 0.02 | − 0.58 | 0.00 | 5.52 | 922.01 |
5 | VRIGAAANS | Chymotrypsin, Trypsin K, IodosoBenzoate, Proline Endopept, Staph Protease, Cyanogen Bromide, AspN | M | NT | A | − 0.07 | − 0.10 | 1.00 | 9.72 | 857.97 |
6 | VYKLQPLTF | AspN, Trypsin R, Clostripain, Staph Protease, Cyanogen Bromide, IodosoBenzoate | NM | NT | NA | 0.02 | − 0.79 | 1.00 | 8.56 | 1108.35 |