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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: HO-1 promotes resistance to an EZH2 inhibitor through the pRB-E2F pathway: correlation with the progression of myelodysplastic syndrome into acute myeloid leukemia

Fig. 2Fig. 2Fig. 2

JQEZ5 significantly promotes MDS cell apoptosis. aThe basic information of MDS patients (patient 3 and patient 7) is shown in Table 2. We selected two typical MDS patients (patient 3 and patient 7) for subsequent experiments. They were both determined to have high expression of EZH2 and HO-1 in previous experiments. The control groups (Con) were not treated with JQEZ5. We prepared different concentrations of JQEZ5, and then cultured cells isolated from the two patients with different concentrations of JQEZ5 for 24 h. We used flow cytometry to detect apoptosis rates and perform comparisons. The bar graph indicates the percentage of annexin V-positive cells (apoptotic cells). b We used flow cytometry to detect the cell cycle distribution of the above experimental groups. The bar graph shows the percentages of cells in different phases of the cell cycle. c HO-1 expression in patient 3 cells was downregulated by Znpp, and HO-1 expression in patient 7 cells was upregulated by Hemin. The patient 7 cells were divided into Con (control), Znpp (treated with 15 μmol/ml ZnppIX), JQEZ5 (treated with 9 μmol/ml JQEZ5) and Znpp + JQEZ5 (treated with 15 μmol/ml ZnppIX and 9 μmol/ml JQEZ5) groups. The patient 3 cells were divided into Con (control), Hemin (treated with 10 μmol/ml Hemin), JQEZ5 (treated with 9 μmol/ml JQEZ5) and Hemin + JQEZ5 (treated with 10 μmol/ml ZnppIX and 9 μmol/ml JQEZ5) groups. d The cell cycle distribution of the above experimental groups were determined. The cell cycle distribution of each group was analyzed by flow cytometry. The Con groups were not treated with any drug. All the experiments were repeated, *P < 0.05 (statistically significant)

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