Circulating microRNAs as minimal residual disease biomarkers in childhood acute lymphoblastic leukemia

Table 7 Associations between miR expression changes and MRD parameters or risk factors

miR	Time points	Fold change	Independent variable	Patient subgroup	Pearson’s r	Adjusted p
miR-128-3p	Day 8 vs day 0	0.107	Flow cytometry MRD in day 15 bone marrow	Whole cohort	0.88	2.71*10⁻⁴
miR-222-3p	Day 8 vs day 0	0.267	Flow cytometry MRD in day 15 bone marrow	Whole cohort	0.81	2.99*10⁻³
miR-128-3p	Day 8 vs day 0	0.149	Flow cytometry MRD in day 15 bone marrow	Normal karyotype^a	0.99	7.53*10⁻⁴
miR-222-3p	Day 8 vs day 0	0.270	Flow cytometry MRD in day 15 bone marrow	Normal karyotype^a	0.97	2.74*10⁻²
miR-222-3p	Day 8 vs day 0	0.190	Absolute peripheral blast count on day 8	t(12;21)	0.96	4.63*10⁻²
miR-181b-5p	Day 33 vs day 0	0.061	Absolute lymphocyte count on day 33	Normal karyotype^a	0.95	2.98*10⁻²
miR-222-3p	Day 33 vs day 0	0.156	Absolute lymphocyte count on day 33	Normal karyotype^a	0.94	3.87*10⁻²

In these analyses, the dependent variable is the normalized miR expression change between the time points compared, all measured in PB PFP. Fold change indicates change in the normalized miR expression between the two time points, the median of these values are provided. Pearson’s r indicates the correlation between the dependent and the independent variables. Adjusted p refers to the statistical significance of the Pearson’s correlation
^aNormal karyotype: no alteration on FISH, DNA index diploid and cytogenetics normal or unsuccessful