Fig. 5

Bradykinin receptor and protein expression. B1-kinin receptor (B1R) and B2-kinin receptor (B2R) expression was analyzed by Western blotting. Protein extracts were obtained from wild control type mice (WT) and from mice with tibial fracture, samples from the fractured side (F) and contralateral non-fractured (NF) side were analyzed. Proteins extracts from hematoma were compared. Tissue sample from B1R and B2R deficient mice were used to check the antibody specificity. The specific of both B1R and B2R antibodies was attested by the absence of protein obtained from B1R and B2R KO mice. Qualitative differences in protein expression of B1R and B2R were shown and were further quantified by densitometry. Results shown in bar graphs are presented as arbitrary units by comparison to expression in WT taken as control. *P < 0.01 when compared to expression in WT