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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: PYCR1 interference inhibits cell growth and survival via c-Jun N-terminal kinase/insulin receptor substrate 1 (JNK/IRS1) pathway in hepatocellular cancer

Fig. 2

The cell proliferation and cell apoptosis influenced by PYCR1 interference in BEL-7404 and SMMC-7721 cells. a, b shPYCR1 group suppressed PYCR1 expression in both mRNA and protein levels. GAPDH was as the internal standard. c Celigo detection. PYCR1 interference significantly inhibited cell proliferation in both BEL-7404 and SMMC-7721 cells. d MTT assay. Cell growth was slower in shPYCR1 group than shCtrl group. On 5th day, the cell count was significantly smaller in shPYCR1 group than shCtrl group in both BEL-7404 and SMMC-7721 cells (P < 0.001). e Colony formation assay. PYCR1 interference significantly decreased the colony number in BEL-7404 and SMMC-7721 cells (P < 0.001). f Apoptosis assay. PYCR1 interference significantly increased the percentage of apoptosis cells in both BEL-7404 (P = 0.004) and SMMC-7721 cells (P = 0.0016). N = 3. Values were expressed as mean ± standard deviation

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