Fig. 2

Effects of ERs silencing on NSCLC cell migration, invasion and apoptosis. a–c PC9/G cells were transfected with si-NC or si-ERs for 24 h and then treated without or with Gefitinib (20 μM) for 48 h. a Cell migration and b invasion capacity were measured by Transwell assays. c Cell apoptosis amount was determined by flow cytometry analysis. d–f PC9/G cells were transfected with si-NC or si-ERs for 48 h. The relative expression levels of ERα and ERβ (d), migration and invasion associated proteins (e), apoptosis associated proteins (f) were analyzed by western blot. All experiments were repeated at least three times. p-values vs. si-NC were estimated using two-tailed unpaired Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001