Fig. 7
Pravastatin increases superoxide production in C2C12 myotubes. a Representative images showing in situ superoxide production as detected by the oxidation of dihydroethidium to fluorescent products (DHE; red fluorescence). b Quantification of DHE fluorescent products. c Quantification of the H2O2 released from cells using the Amplex Red probe during a 1-h incubation (areas under the curves). Mean ± SE, two independent experiments with six replicates in each experiment. *p < 0.05 as determined by one-way analysis of variance (ANOVA) with LSD (Fisher’s least significance difference) post hoc test